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KMID : 1144820180240010015
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대한의생명과학회지
2018 Volume.24 No. 1 p.15 ~ p.22
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Pristimerin, a Naturally Occurring Triterpenoid, Exerts Potent Anticancer Effect in Colon Cancer Cells
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Seo Hee-Won
Park Ju-Hyung
Lee Ji-Yeon
Park Hyun-Ju
Kim Jin-Kyung
Park Ju-Hyung
Lee Ji-Yeon
Park Hyun-Ju
Kim Jin-Kyung
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Abstract
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Pristimerin is a triterpene compound isolated from plant extracts that reportedly possesses antitumor, antioxidant, and anti-inflammatory activities. The current study was designed to evaluate the antitumor effects of pristimerin on human colon cancer cells. Treatment of the human colon cancer cells, HCT116 and SW480, with pristimerin led to a dose-dependent decrease in cell proliferation. Flow cytometry experiments showed that pristimerin increased cell apoptotic rate and decreased the mitochondrial membrane potential in HCT116 and SW480 cells. Western blot assay showed that pristimerin induced increased cleavage of caspase-3, -7, -8, and poly ADP ribose polymerase. Treatment with pristimerin also caused a marked decrease in the expression of Bcl-2 and Bcl-xL. Additionally, the levels of phosphorylated AKT and forkhead box O3a (FOXO3a) were decreased in pristimerin-treated colon cancer cells. Taken together, our study illustrated that pristimerin promoted apoptosis via the AKT/FOXO3a signaling pathway in colon cancer cells, elucidating that it might be considered as a potential agent for colon cancer therapy.
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KEYWORD
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Apoptosis, AKT, Colon cancer cells, Forkhead box O3a, Pristimerin
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